Neurochemical development of the hippocampal region in the fetal rhesus monkey. III: Calbindin-D28K, calretinin and parvalbumin with special mention of cajal-retzius cells and the retrosplenial cortex

Abstract Ten adult male chinchillas were used. The localisation of calbindin D28k (CB) was examined with the use of two types of reactions: immunocytochemical peroxidase-antiperoxidase and immunofluorescence staining with a specific monoclonal antibody against CB. Immunocytochemical examination demonstrated the presence of CB-positive neurons in the following layers of all parts the parahippocampal gyrus (PG): marginal, external cellular, middle cellular, and internal cellular, i.e. in entorhinal area, parasubiculum, and presubiculum. Immunofluorescence staining revealed the presence of CB in both Hu C/Dimmunoreactive (IR) neurons and nervous fibers of the PG. CB-IR neuronal cell bodies were moderately numerous (ca. 10% of Hu C/D-IR neurons) and clearly distinguished from the background. Each layer of the brain area consisted of two types of neurons: pyramidal and multiform. Among the second type of neurons, four kinds of morphologically different neuronal subclasses were observed: multipolar, bipolar, round, and Cajal-Retzius cells. It is concluded that the expression of CB in the PG of the chinchilla is species specific and limited to several subclasses of neurons

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The time of origin of neurons in the hippocampal region of the rhesus monkey

The Journal of Comparative Neurology ◽

10.1002/cne.901960109 ◽

1981 ◽

Vol 196 (1) ◽

pp. 99-128 ◽

Cited By ~ 218

Author(s):

P. Rakic ◽

R. S. Nowakowski

Keyword(s):

Rhesus Monkey ◽

Hippocampal Region ◽

Time Of Origin

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Retrosplenial cortex in the rhesus monkey: A cytoarchitectonic and golgi study

The Journal of Comparative Neurology ◽

10.1002/cne.901690105 ◽

1976 ◽

Vol 169 (1) ◽

pp. 63-97 ◽

Cited By ~ 95

Author(s):

Brent Alan Vogt

Keyword(s):

Rhesus Monkey ◽

Retrosplenial Cortex ◽

Golgi Study

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Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

Reproduction ◽

10.1530/rep.1.00226 ◽

2004 ◽

Vol 128 (4) ◽

pp. 433-441 ◽

Cited By ~ 27

Author(s):

Kien C Luu ◽

Gui Ying Nie ◽

Anne Hampton ◽

Guo-Qiang Fu ◽

Yi-Xun Liu ◽

...

Keyword(s):

Rhesus Monkey ◽

Blot Analysis ◽

Embryo Implantation ◽

Pcr Analysis ◽

Uterine Receptivity ◽

Calbindin D9k ◽

Evolutionary Changes ◽

Ef Hand ◽

Calbindin D28k ◽

Specific Regulation

The endometrium is hostile to embryo implantation except during the ‘window of receptivity’. A change in endometrial gene expression is required for the development of receptivity. Calbindin-d9k (CaBP-d9k) and calbindin-d28k (CaBP-d28k) are proteins possessing EF-hand motifs which have high affinity for Ca2+ ions. Previously, it has been demonstrated that, in mouse endometrium, the expression of both calbindins is highly regulated during implantation and that both proteins play critical but functionally redundant roles at implantation. This study was the first to determine the expression of these two calbindins in the human and rhesus monkey endometrium. Initial RT-PCR analysis demonstrated that CaBP-d28k but not CaBP-d9k mRNA expression is detectable in the endometrium of both species. Western blot analysis confirmed the presence of immuno-reactive CaBP-d28k protein in the primate endometrium. Furthermore, the endometrial expression pattern of CaBP-d28k mRNA and protein was examined by Northern blot analysis and immunohistochemistry respectively in both species across the menstrual cycle and during early pregnancy. Semi-quantitative statistical analysis of the immunohistochemistry results revealed that, in the human, CaBP-d28k protein expression was maximal in luminal and glandular epithelium during the mid-secretory phase, coinciding with the time when the endometrium is receptive to embryo implantation. Expression in rhesus monkey showed a similar trend. These results suggest that, in the primate endometrium, only CaBP-d28k is expressed and that the specific regulation of this calbindin is potentially important for the establishment of uterine receptivity.

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